Here is a list of the most recent publications from our lab :

Tucker, B.A., Hudson, J.S., Ding, L., Lewis, E., Sheardy, R.D., Kharlampieva, E., and Graves, D. (2018) “Stability of the Na+ Form of the Human telomeric G-Quadruplex: Role of Adenines in Stabilizing G-Quadruplex Structure”, ACS Omega, 3 (1), 844-855. PDF (2065 KB)

Mikek, C.G., Machha, V.R., White, J.C., Martin, L.R., West, S.J., Butrin, A., Shumaker, C.L., Gwin, J.C., Alatrash. N., MacDonnell, F.M., and Lewis, E.A. (2017) “The Thermodynamic Effects of Ligand Structure on the Molecular Recognition of Mono- and Bi-Ruthenium Polypyridyl Complexes with G-quadruplex DNA” Eur. J. Inorg. Chem., (33), 3593-3960. PDF (2199 KB)

Machha, V.R., Mikek, C.G. Wellman, S. and Lewis, E.A. (2017) “Temperature and Osmotic Stress Dependence of the Thermodynamics for Binding Linker Histone H10, Its Carboxyl Domain (H10-C) or Globular Domain (H10-G) to B-DNA”, Biochemistry and Biophysics Reports (12), 158-165. PDF (921 KB)

Mikek, C.G., DuPont, J., Machha, V.R., White, J.C., Martin, L.R., Alatrash. N., MacDonnell, F.M., and Lewis, E.A. (2017) “The Thermodynamic Effects of Ligand Structure on the Molecular Recognition of Mononuclear Ruthenium Polypyridyl Complexes with B-DNA”, European Journal of Inorganic Chemistry (30), 3604-3611. PDF (928 KB)

Henderson, K.L., Li, M., Martinez, S., Lewis, E.A., Hausinger, R.P., and Emerson, J.P. (2017) “Global stability of an α-ketoglutarate-dependent dioxygenase (TauD) and its related complexes.” Biochim. Biophys. Acta, 1861, 987-994.PDF (768 KB)

Wang, C.; Carter-Cooper, B.; Du, Y.; Zhou, J.; Saeed, M. A.; Liu, J.; Guo, M.; Roembke, B.; Mikek, C.; Lewis, E. A. (2016) “Alkyne-substituted diminazene as G-quadruplex binders with anticancer activities.” European Journal of Medicinal Chemistry 2016. Aug 8. doi:10.1016/j.ejmech.2016.04.030 PDF (1360 KB)

Henderson, K. L.; Boyles D. K.; Le, V.H.; Lewis E. A.; and Emerson J. P. (2016) “ITC Methods for Assessing Buffer/Protein Interactions from the Perturbation of Steady-State Kinetics: A Reactivity Study of Homoprotocatechuate 2,3-Dioxygenase.” Methods in Enzymology (567), 257-78. PDF (1875 KB)

DuPont, J. I.; Henderson, K. L.; Metz, A.; Le, V. H.; Emerson, J. P., and Lewis E. A. (2015) “Calorimetric and spectroscopic investigations of the binding of metallated porphyrins to G-quadruplex DNA.” Biochim Biophys Acta 2015 Sep 9. pii: S0304-4165(15)00235-4. PDF (1378 KB)

Henderson, K. L.; Francis, D. H.; Lewis E. A.; and Emerson J. P. (2015) “Thermodynamics of substrate binding to the metal site in homoprotocatechuate 2,3-dioxygenase: Using ITC under anaerobic conditions to study enzyme–substrate interactions.” Biochim Biophys Acta 2015. Aug 23. pii: S0304-4165(15)00210-X. PDF (871 KB)

Le, V. H.; McGuire, M.; Ahuja, P.; MacDonnell, F.; and Lewis E. A. (2015) “Thermodynamic investigation of [(phen)₂Ru(tatpp)Ru(phen)₂]⁴⁺ interactions with B-DNA.” Journal of Physical Chemistry, Part B, 119(1), 65-71. PDF (1017 KB)

Le, V. H.; Yanney, M.; McGuire, M.; Sygula, A.; and Lewis, E. A. (2014) “Thermodynamics of Host-Guest Interactions Between Fullerenes and a Buckycatcher”. Journal of Physical Chemistry, Part B, 118(41), 11956-11964. PDF (1708 KB)

Zhou, J.; Le, V. H.; Kalia, D.; Nakayamaa, S.; Mikek, C.; Lewis, E. A.; and Sintim, H. O. (2014), “Diminazene or berenil, a classic duplex minor groove binder, binds to G-quadruplexes with low nanomolar dissociation constants and the amidine groups are also critical for G-quadruplex binding.” Molecular BioSystems. 10,2724-2734. PDF (2649 KB)

Hudson, J. S.; Ding, L.; Le, V.; Lewis, E. A.; and Graves, D. E. (2014), “Recognition and Binding of Human Telomeric G-Quadruplex DNA by Unfolding Protein 1 (UP1)”. Biochemistry, 53(20), 3347-3356 PDF (1473 KB)

Lyons, D. F.; Le, V.; Kramer, W. H.; Bidwell, G.; Lewis, E. A.; Raucher, D.; and Correia, J. J. (2014), “The effect of basic cell penetrating peptides on the structural, thermodynamic and hydrodynamic properties of a novel drug delivery vector: ELP[V5G3A2-150]”. Biochemistry, 53(6), 1081-1091. PDF (2010 KB)

Machha, V.R, Jones, S.B., Waddle, J.R., Le, V.H., Wellman, S., and Lewis, E.A. (2014), “Exploring the Energetics of Histone H1.1 and H1.4 Duplex DNA Interactions”. Biophysical Chemistry, 185, 32-38. PDF (422 KB)

Song, H.; Weitz, A. C.; Hendrich, M. P.; Lewis, E. A.; and Emerson, J. P. (2013) “Building reactive copper centers in human carbonic anhydrase II.” Journal of Biological Inorganic Chemistry. 18(6): 595–598. PDF (255 KB)

Ramos, J. P.; Le, V. H.; and Lewis, E. A. (2013), “Role of Water in Netropsin Binding to an A₂T₂ Hairpin DNA Site: Osmotic Stress Experiments”. Journal of Physical Chemistry, Part B,117 (50), 15958-15965. PDF (432 KB)

Cui, J.; Waltman, P.; Le, V. H.; and Lewis, E. A. (2013), “The Effect of Molecular Crowding on the Stability of Human c-MYC Promoter Sequence i-Motif at Neutral pH”. Molecules, 18, 12751-12767.  PDF (422 KB)

Rowland, G. B.l Barnett, K.; DuPont, J. I.; Akurathi, G.; Le, V.H.; and Lewis, E. A. (2013), “The Effect of Pyridyl substitution on the Thermodynamics of Porphyrin Binding to G-quadruplex DNA”. Bioorganic & Medicinal Chemistry, 21, 7515-7522.PDF (1051 KB)

Machha, V. R.; Waddle, J.; Turner, A.; Wellman, S.; Le, V. H.; and Lewis, E. A. (2013) “Calorimetric Studies of the Interaction of Linker Histone H10 and its Carboxyl (H10-C) and Globular (H10-G) Domains with Calf-Thymus DNA”. Biophysical Chemistry, 184, 22-28. PDF (801 KB)

Le, V.H.; Nagesh, N.; and Lewis, E.A. (2013) “Bcl-2 Promoter Sequence G-Quadruplex Interactions with Three Planar and Non-Planar Cationic Porphyrins, TMPyP4, TMPyP3, and TMPyP2”. PLOSONE, 8(8): e72462. DOI:10.1371/journal.pone.0072462 PDF (716 KB)

Lyons, D.F.; Le, V.; Bidwell, G.L.; Kramer, W.H.; Lewis, E.A.; Raucher, D.; and Correia, J.J.(2013) “Structural and Hydrodynamic Analysis of a Novel Drug Delivery Vector: ELP[V5G3A2-150]”. Biophysical Journal, 104(9) 2009-2021. PDF (783 KB)

Le, V. H.; Buscaglia, R.; Chaires, J. B.; and Lewis, E. A. (2013), “Modeling Complex Equilibria in ITC Experiments: Thermodynamic Parameters Estimation for a Three Binding Site Model”, Analytical Biochemistry, 434(2) 233-241. PDF (1294 KB)

Emerson,J. P.; Le, V.; and Lewis, E. A. (2012) “Calorimetry”, eLS, John Wiley and Sons Ltd, DOI: 10.1002/9780470015902.a0003010.pub3. PDF (600 KB)

Song, H.; Wilson, D.L.; Farquhar, E.R.; Lewis, E.A.; and Emerson, J. P. (2012) “Revisiting Zinc Coordination in Human Carbonic Anhydrase II”, Inorganic Chemistry, 51(20) 11098-11105. PDF (1167 KB)

Henderson, K. L.; Le, V. H.; Lewis, E. A., and Emerson, J. P. (2012) “Exploring substrate binding in homoprotocatechuate 2,3-dioxygenase using isothermal titration calorimetry”, JBIC, 17(7) 991-994. PDF (310 KB)

Vangala, K.; Ameer, F.; Salomons, G.; Le, V.; Lewis, E. A.; Liu, D.; Yu, L.; Zhang, D. (2012). “Studying Protein and Gold Nanoparticle Interaction Using Organothiols as Molecular Probes”, Journal of Physical Chemistry C., 116(5), 3645-3652. PDF (2824 KB)

Dettler, J. M. and Lewis, E. A. (2011) “Biophysical Studies of the Structure, Stability and Ligand Properties of G-Quadruplex DNA: Thoughts and Comparisons of the K-ras, c-MYC, and Bcl-2 Oncogene promoter Sequence Quadruplexes”, Chapter 3, pp 33-50 in ACS Symposium Series, v 1082, Frontiers in Nucleic Acids, R.W. Sheardy and S.A. Winkle, eds. Doi:10.1021/bk-2011-1082.ch003, published online October 25,2011. Link out

Lewis, E. A.; Munde, M.; Wang, S.; Rettig, M.; Le, V.; Machha, V.; and Wilson, W. D. (2011) “Complexity in the binding of minor groove agents: netropsin has two thermodynamically different DNA binding modes at a single site”, Nucleic Acids Research, 39(22) 9649-9658. PDF (832 KB)

Dettler, J. M.; Buscaglia, R.; Le, V.; and Lewis, E. A. (2011) “DSC Deconvolution of the Structural Complexity of c-MYC P1 Promoter G-Quadruplexes”. Biophysical Journal, 100(6),1517-1525. PDF (378 KB)

Dettler, J. M.; Buscaglia, R.; Cui, J.; Cashman, D.; Blynn, M.; and Lewis, E. A. (2010) “Biophysical Characterization of an Ensemble of Intramolecular i-Motifs Formed by the Human c-MYC NHE III1 P1 Promoter Mutant Sequence”. Biophysical Journal, 99(2) 561-7. PDF (370 KB)

Nagesh, N.; Buscaglia, R.; Dettler, J. M.; and Lewis, E. A. (2010) “Studies on the Site and Mode of TMPyP4 Interactions with Bcl-2 Promoter Sequence G-quadruplexes”. Biophysical Journal, 98(11) 2628-33. PDF (384 KB)

Nagesh, N.; Sharma, V. K.; Kumar, A. G.; and Lewis, E. A. (2009) “Effect of Ionic Strength on Porphyrin Drug Interactions with Quadruplex DNA Formed by the Promoter Region of c-MYC and Bcl-2 Oncogenes”. Journal of Nucleic Acids, v. 2010, Article ID 146418, 9 pages, DOI:10.4061/2010/146418. PDF (709 KB)

Lewis, E. A. and Murphy, K. P. (2009) “Calorimetry”. Encyclopedia of Life Sciences, Article Online Posting Date: December 15, 2009, DOI: 0.1002/9780470015902.a0003010.pub2. PDF (375 KB)

Cashman, D., Buscaglia, R.; Freyer, M. W.; Dettler, J.; Hurley, L. H.; and Lewis, E. A. (2008) “Molecular Modeling and Biophysical Analysis of the c-MYC NHE-III1 Silencer Element”. Journal of Molecular Modeling, 14(2), 93-101. PDF (396 KB)

Smith, R. F.; Freyer, M. W.; and Lewis, E. A. (2007) “Biophysical Characterization of Vaccinia Virus Thymidine Kinase Substrate Utilization”. Journal of Virological Methods, 142, (1-2), 151-158. PDF (309 KB)

Freyer, M. W.; Buscaglia, R.; Hollingsworth, A.; Ramos, J. P.; Blynn, M.; Pratt, R.; Wilson, W. D.; and Lewis, E. A. (2007) “Break in the Heat Capacity Change at 303 K for Complex Binding of Netropsin to AATT Containing Hairpin DNA Constructs”. Biophysical Journal, 92, 2516-2522. PDF (124 KB)

Freyer, M. W.; and Lewis, E. A. (2007) “Isothermal Titration Calorimetry: Experimental Design, Data Analysis and Probing Macromolecule/Ligand Binding and Kinetic Interactions”. Chapter 4 in Biophysical Tools for Biologists Vol 1, (Methods in Cell Biology, v. 84), ed. Correia, JJ, Detrich, HW, Academic Press, San Diego, CA, 79-113. PDF (354 KB)

Freyer, M. W.; Buscaglia, R.; Kaplan, K.; Cashman, D.; Hurley, L. H.; and Lewis, E. A. (2007), “Biophysical Studies of the c-MYC NHE III1 Promoter: Model Quadruplex Interactions with a Cationic Porphyrin”. Biophysical Journal, 92(6), 1-9. PDF (190 KB)

Freyer, M. W.; Buscaglia, R.; Cashman, D.; Hyslop, S.; Wilson, W. D.; Chaires, J. B.; Lewis, E. A. (2007), “Binding of Netropsin to Several DNA Constructs: Evidence for at Least Two Different 1:1 Complexes Formed from an A2T2 Containing ds-DNA Construct and a Single Minor Groove Binding Ligand”. Biophysical Chemistry, 126, 186-196. PDF (1129 KB)

Freyer, M. W.; Buscaglia, R.; Nguyen, B.; Wilson, W. D.; Chaires, J. B.; and Lewis, E. A. (2006), “Binding of Netropsin and DAPI to an A2T2 DNA Hairpin: A Comparison of Biophysical Techniques”. Analytical Biochemistry, 355, 259-266. PDF (628 KB)

Lewis, E. A. and Murphy, K.P. (2005) “Isothermal Titration Calorimetry”, Methods in Molecular Biology, vol 305: Protein-Ligand Interactions: Methods and Applications”. Humana Press, Inc. Totowa, NJ, 1-16 . Link out

Horn, J. R.; Russell, D.; Lewis, E.A.; and Murphy, K. P. (2001) “van’t Hoff and Calorimetric Enthalpies from Isothermal Titration Calorimetry: Are there Significant Discrepancies?”. Biochemistry, 40, 1774-1778. PDF (59 KB)